At least four people were wounded, two of them in critical condition, during a protest that took place in Hadramout province.
The protesters took to the streets in several districts chantingd anti-government slogans, and demanded the release of detainees of Sothern Movement.
In return, security forces used tear gas and gun fire to disperse the protesters before they arrested dozens of members of Sothern Movement during the protest.
The main leaders of the Southern Movement, Ali Salem Al-Baid, who is in exile, and Hassan Baoum called for Tuesday to be a day of rage and protest against the Yemeni government coinciding with the opposition rallies in Yemeni provinces.