Human rights activists, journalists, academics and politicians, who staged a sit-in Tuesday at the Al-Hurriya Square coinciding with the cabinet's weekly meeting, urged the government to step down.
The government must submit its resignation and at once, the sit-inners said.
During the protest, mainly in solidarity with those who were arrested in the south and kidnapped teens, head of the Supreme Opposition Council Sultan Al-Etwani accused the regime of pushing the people for secessionism as he urged further sit-ins until legal demands are met.
The current regime is behind the deteriorating situation in the country and its acts are driving the people to the wall, he said, pointing to those calling for the separation of the south.
Amid the latest developments the people no longer think of unity and prosperity, he added.
Al-Etwani called on the ministers to come back to their homes as they have proved unable to bear sense of responsibility towards the nation.
For his part, secretary general of the Yemeni Medical Doctors' Syndicate Abdul Qawi Al-Shamiry depicted the ministers as the regime's slaves.
The HOOD urged General Attorney to investigate the recent events in the south and pursue criminals.
Head of the organization Mohammed Naji Alaw, who led a demonstration to the Cabinet Presidency and the Attorney General's office, said attacks against rallies at the Al-Hashmi Square, Aden, were deemed as attacks against the constitution and stifling the people's freedom.
He urged to investigate the attacks.
Other participants called for fair treatment of the people who demand better life and more rights.
As unrest intensified in the south with the people fighting the troops and calling for the separation of the south, many demonstrations have been cracked down, with hundreds arrested.