At least seven people were wounded, three of them soldiers, during a protest that took place in Aden province on Wednesday.
The protesters took to the streets in several Aden neighborhoods and chanted anti-government slogans. They set fire on car tires, blocked several roads and wounded a soldier when they hurled stones at security forces, sources said.
In return, security forces used tear gas and gun fire to disperse the protesters before they arrested dozens of members of Southern Movement during the protest.
On Tuesday, dozens of other protesters were arrested during clashes that erupted between Yemeni security forces and protesters in Aden.
The main leaders of the Southern Movement, Ali Salem al-Baid, who is in exile, and Hassan Baoum called for Tuesday to be a day of rage to protest against the Yemeni government.